Product killed hog cholera virus and process of producing it



Patented Mar. 6, 1 928 ma amount, or unusual; rn'nnsinvnnin;

rnonuc'r Kitten oG-oHonnnA VIRUS nnnrnoonss or rnonuorns rr! I No Drawing.

The invention relates to anew andusful product :killed hog cholera virus, i andprocess for producing it, which product .1

can be used alone or with'anti-hog-cholera serum as an immunizing element of danger or risk of disease of hog cholerain susceptible'hogs.

In carrying out the process, the'cause of hog cholera in whatever available form, or

10 live virulent hogcholera virus, in the formof virulent blood from a hog infected or sick of hog cholera, or live virulent virus serum, or de-;

when injected in SllflIlOlBIlt doses asdescribed p hereinafter. g Living hog cholera virus inia highly virulent form has been used for many years in the simultaneous treatment with anti-hog cholera serum in' the immunization of hogs, andliving hog cholera virus has beenexperimented with with a view of attenuating its virulence, or'infectivity;

but no eflort has as yet beenmade to kill hog choleravirus with the view or object of determining or.

establishing its immunizing value in 'sufficient doses as described hereinafter and which may be injected in sufficient doses as described herein simultaneously with anti-' hog cholera serum or without said serum.

' -My process, as

40 amount of live virulent hog cholera virus in the form of-defibrinated-blood, and adding thereto a preservative, forv instance, such as 0.5% phenol, and placing the mixture-fin a refrigerator at a temperature of fromv 5 to 10 C..for a period suificiently long to'check all bacterial growth, say, approximately from thirty to ninety days, and thensubhigher temperature (1., preferably however a v jecting the mixture-to a than 5 to 10 temperature that is not so high as will cause physical changes'to occur therein that would detract from the usefulness ofthe product. An exposure of the mixture to 37 C; or thereabouts for a periodactually to killed all bacterial contamination,

agent without the causing the j ected with subsequent injection of 2 cc; living hereinafter disclosed, is, preferably carried out by taking a definite b pp mately thirty days will also kill the hog Application flied February-16,1925, f seriallj'o', 9,706.

or non-infective, Wheninjected into susceptible hogs insufficientdoses of from 5 cc. to i 50 cc. 'for each fifty pounds or" fraction thereof of hog. The injection of the killed virus in the suflicient dosage as above 'de-" scribed into susceptible hogs regardless of their sizes will beunaccompanied by the development of hog cholera in the hogginjected, and the same hogs will be subse-' quently found-toabe immune to any. natu- 'ral nfectionof hog cholera, livev virulent hog cholera virus; ,provi ded the injections were-1n suificient dosages'as described above and suflicient time has been granted to permit the hogsinjected with the I killed hogcholera virus in sufficient-dosage to the injectionand develop the increased resistance or immuni as above; given to react ty. It; has been demonstrated that hogs invirulent hog cholera virus, thereby proving that only killed hog cholera virus jected.

.Havi'ng disclosed apractical way of killing live, virulent hog cholera virus by chem above described 1 ical or physical means as and producing the'product, killed hog cholera virus, and having jectjion in sufficient. and test of my killed hog cholera virus? proddosage as described above to not that I obtain by my pr'ocess asdescribed g cholera virusfthus'making it non-virulent, V i

or injection of o h the above described-killedvirus roduct in sufiicient dosage" as. described above andheld six weeks will withstand a I 7S0 was" originally indemonstrated by inherein and itsimmunizlng value can be. re-

lied upon when injected in as. described above and properly used, v

What I'vclaim and desire to protect by Letters Patent is:" ,1. The'process' cholera virus from live virulent hog cholera vir us' consisting in adding a preservative c to live virulent hog cholera virus, and subjecting the mixture C "for a period only, long of obtaining kill d hog to a temperaturev of enough'to check bacterial growth, andthen w exposing it to a temperature of about 37 C. for a period only long enough to kill all bacterial contamination virus. r

2. The process nd the hog cholera of obtaining killed hog cholera virus, consistingin adding to a definite quantity of live virulent hog cholera virus in the form of defibrinated blood 05% phenol, and subje'c ingthe mixture to a tem perature of from 5 to- 10 C. for from thirty to ninety days, sufficient to" check hacterial growth, and then a'temperature cf about 37 C. for about thirty days, to kill all bacterial contamination and the hog 'cholera viruse 3. Killed hog cholera 'viru's clisscciat'ecl from live virulent hog cholera virus, as a noninfective and immunizing agent against hog cholera when .lIlJBCtGCl into susceptible hogs in doses cf from'5 cc. to 50 ccpfor each {lth day of February 

